The genomic locations of SYGGRG, motif 2, and motif 4 are indicated on the genome browser by red, blue, and purple markings, respectively. (F) Genome browser screenshots showing CreA ChIP-seq signals at representative CreA binding sites with or without the SYGGRG motif. Background ChIP-seq signal in an untagged wild-type strain grown under glucose conditions was included as a control. (E) A boxplot displaying CreA ChIP-seq signals at CreA binding sites with or without SYGGRG motif. (D) A diagram showing the frequency of SYGGRG motif across 1-kb regions spanning CreA binding sites. The signals across a 3-kb window spanning each CreA ChIP-seq summit are displayed. (C) Heatmaps displaying CreA HA and CreA GFP ChIP-seq and background (WT) signals over CreA binding sites identified in the CreA HA and CreA GFP strains. (B) A Venn diagram showing the overlap of CreA binding sites detected in CreA HA and CreA GFP strains. Annotated genes are indicated by gray lines at the bottom. ChIP-seq signals in nontagged wild-type strains are presented as negative controls for the corresponding ChIP-seq experiments. (A) A genome browser view of CreA HA (in blue) and CreA GFP (in green) ChIP-seq signals over chromosome III of A.
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